Description:

E.coliUvrDisaDNAhelicasethatusestheenergygeneratedbythehydrolysisofnucleosidetriphosphatestobreakthehydrogenbondslinkingthetwostrandstogetherinduplexDNA.Itisactiveat37ºC.

SUPPLIEDWITH:
10XHelicaseBufferM


SuppliedbyUsers:ATP

Source:

Reactioncondition:

1XHelicaseBufferM:
10mMDithiolthreitol
10mMMagnesiumAcetate
35mMTris-AcetatepH7.5@25°C

3mMATP.
Incubateat37ºC.

Notes:
E.coliUvrDhelicasemayrequireMutLand/orSSBforoptimalperformanceinspecificapplications.

:MutLstimulatesUvrDhelicaseactivitymorethantenfoldbyloADIngitontotheDNAsubstrate(MechanicLE,FrankelBA,MatsonSW(2000)JBiolChem275:38337–38346).

:MutLandSSBarerequiredinHelicase-DependentAmplificationusingE.coliUvrD(MyriamVincent,YanXu,andHuiminKong(2004)EMBOreportsVol.5,795-800).

StorageConditions:

StorageTemperature:
-20ºC

Concentration:
0.5mg/ml

StorageBuffer:
20mMTris-HClpH8.3
200mMNaCl
1.5mMEDTA
25mMß-Mercaptoethanol
50%Glycerol

QualityControl:

QualityAssuranceStatement:
E.coliUvrDHelicaseispurifiedfreeofcontaminatingendonucleasesandexonucleases.EachlotistestedforDNAunwindingactivity.

Biohelix

自2007年以来，生物螺旋公司一直致力于为世界各地的生命科学研究人员提供最好的生物试剂。我们的产品包括DNA标记、蛋白质标记、预铸蛋白凝胶、核酸纯化、PCR试剂等。

Bio-Helix has been focusing on providing the best bio-reagents for life science researchers all over the world since 2007. Our products, to name a few, include DNA markers, protein markers, pre-cast protein gel, nucleic acid purification, PCR reagents and so on. Working together with scientists in the lab around the world and continuing efforts of quality improvement, we begin to gain trusts and appraises from customers. The brand, GeneDirex, spreading in more than forty countries, has become a trustworthy and welcome brand of reagents in laboratories of life science.